Web Tools for Advanced PCR Design, Genotyping, LAMP and Sequence Analysis

Browse Tools

Search and open tools. All pages are optimized for modern browsers and mobile screens.

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Design & Assays

PCR, Multiplex, and QF-PCR primer design

Advanced capabilities for designing primers: standard, inverse, multiplex, quantitative fluorescence (TaqMan, MGB-probe), bisulfite PCR and RPA assay design.

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Multiplex Tiling PCR Panel Design

Custom multiplex tiling PCR panel design for NGS and TGS amplicon sequencing. Powerful method for targeted sequencing across organisms.

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KASP Primers Genotyping Assay

Allele-Specific PCR (AS-PCR) - KASP™/PACE™/ASQ-based genotyping assay designs for SNPs and InDels discrimination.

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LAMP Primer Design

Loop-mediated Isothermal Amplification using 4-6 primers recognizing 6-8 distinct regions. Highly sensitive, specific, and rapid DNA amplification.

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Gibson Assembly Primer Design

Isothermal, single-reaction method for assembling multiple overlapping DNA molecules. Seamlessly construct genes, pathways and genomes.

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In Silico PCR Tool

Virtual ePCR for primers/probes, miRNA or gRNA binding site prediction. Eliminates off-target effects and predicts PCR products.

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Oligo Analysis

PrimerAnalyser

Comprehensive analysis: length, GC%, Tm, molecular weight, extinction coefficient, OD, linguistic complexity, and self-dimer detection.

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PrimersList

Analyze multiple primers simultaneously: Tm, GC%, complexity, molecular weight, extinction coefficient, OD, self-dimers and cross-dimers.

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Gator GeneSwift Oligo Probe Design

Simple, fast, and reliable method for AAV titer determination using lysis and genome hybridization with BLI detection.

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Repeats & Genome

TotalRepeats

De novo identification, masking, and clustering of all repeated sequences at the genomic scale. Detects interspersed repeats and SSRs.

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DNA Multiple Sequence Alignment (MSA)

Progressive MSA for DNA (ACGTN) using k-mer distances, UPGMA guide tree, and profile alignment. For small to medium datasets in-browser.

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Calculators

PCR Reaction Setup Calculator

PCR, LAMP or any reaction setup calculator. Calculate reagent volumes for your experiments.

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Universal Dilution Calculator

Mix two solutions with various concentrations (molar, %, or other), different pH levels, or stock solution with solvent.

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