Virtual (in silico) or electronic PCR (ePCR) for primers/probes, miRNA or gRNA binding site prediction. Eliminates"off-target" effects against whole genomes, predicts PCR products and searches for potential mismatches.
Alternative: For unlimited genome sizes, use the command-line in silico PCR Java application.
Retrieve sequence from NCBI by accession
— chromosomes (NC_), mRNA (NM_), RefSeq contigs
Upload local FASTA file
— plain FASTA, multi-FASTA, or sequences
Amplicon Detection Parameters
Need details? Sequence markup, parameters, output columns and the suggested workflow are documented in Help: ePCR tool · Help: Troubleshooting