Analyzes multiple primers: melting temperature (Tm), GC content, secondary structures (hairpins, self-dimers, cross-dimers), linguistic complexity, molecular weight, extinction coefficient, and OD calculations.
Upload or paste sequence(s) in FASTA format:
Input
Features
Dimers
Tm Calculation Parameters
Degenerate codes:
LNA:
B=CGT, D=AGT, H=ACT, K=GT, M=AC, N=ACGT, R=AG, S=GC, V=ACG, W=AT, Y=CT, U=Uracil, I=Inosine.
LNA:
E=dA, F=dC, J=dG, L=dT.
Paste sequences as
name sequence (space/tab separated) or copy from Excel.Output: Export: Select all (Ctrl+A), copy (Ctrl+C), paste (Ctrl+V)
Quick Help
- Paste primer sequences as 'name sequence' (space/tab) or table-like text from Excel.
- Adjust salt/Mg/primer concentration to match your assay conditions before running Analysis.