Molecular Biology Toolbox

Standard, inverse, multiplex, TaqMan, MGB-probe, bisulfite PCR and RPA assay design.

Custom amplicon panel for NGS and TGS targeted sequencing across organisms.

AS-PCR / KASP™ / PACE™ / ASQ genotyping for SNPs, InDels and haplotypes.

Isothermal amplification using 4–6 primers recognizing 6–8 distinct target regions.

Seamless isothermal assembly of multiple overlapping DNA fragments in a single reaction.

Virtual PCR for primers, probes, miRNA or gRNA binding prediction and off-target detection.

Single-oligo analysis: Tm, GC%, MW, extinction coefficient, OD, complexity, self-dimers, G-quadruplexes.

Batch analysis of multiple primers: Tm, GC%, complexity, MW, self-dimers and cross-dimers.

Fluorescein / biotin probe pairs for AAV titer by biolayer interferometry (BLI).

De novo identification, masking and clustering of repeats at genomic scale. Detects SSRs and interspersed repeats.

Needleman–Wunsch, Smith–Waterman and progressive MSA — all in the browser.

Calculate reagent volumes for PCR, qPCR and LAMP. Supports 10+ polymerase presets.

Mix two solutions — molar, %, pH or any unit. Provide 4 of 6 variables; the rest are computed.