Loop-mediated Isothermal Amplification (LAMP) uses 4–6 primers recognizing 6–8 distinct regions of target DNA for highly specific amplification. LAMP reactions occur under isothermal conditions without special thermal cyclers.
Key features: Four core primers + two loop primers for accelerated reaction. DNA products are >20 kb formed from numerous repeats of the 200–350 bp target sequence.
Retrieve sequence from NCBI by accession
— chromosomes (NC_), mRNA (NM_), RefSeq contigs
Upload local FASTA file
— plain FASTA, multi-FASTA, or sequences
Primer Design Options
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- Distance: F2-B2: 120–160 bp; F2-F3 and B2-B3: 0–20 bp; Loop regions: 0–40 bp
- Tm values: F1c/B1c/LF/LB: 64–66°C; F2/B2/F3/B3: 59–61°C
- Region marking: Use
[and]for target;/.../to exclude - IUB/IUPAC codes: B=CGT, D=AGT, K=GT, M=AC, N=ACGT, R=AG, S=GC, W=AT, Y=CT
Output: Export: Select all (Ctrl+A), copy (Ctrl+C), paste (Ctrl+V)
Quick Help for LAMP Primer Sets
- Paste/upload FASTA or retrieve by NCBI accession, then tune length/Tm ranges for your chemistry.
- Use [ ... ] to constrain the target region and /.../ to exclude problematic segments.