Loop-mediated Isothermal Amplification (LAMP) uses 4-6 primers recognizing 6-8 distinct regions of target DNA for highly specific amplification. LAMP reactions occur under isothermal conditions without special thermal cyclers.
Key features: Four core primers + two loop primers for accelerated reaction. DNA products are >20 kb formed from numerous repeats of the 200-350 bp target sequence.
Upload or paste sequence(s) in FASTA format:
Target sequence(s)
Pre-designed list of primers/probes
Report (Primer list)
LAMP Primer Sets
Primer Design Options
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- Distance: F2-B2: 120-160 bp; F2-F3 and B2-B3: 0-20 bp; Loop regions: 0-40 bp
- Tm values: F1c/B1c/LF/LB: 64-66°C; F2/B2/F3/B3: 59-61°C
- Region marking: Use
[and]for target;/.../to exclude - IUB/IUPAC codes: B=CGT, D=AGT, K=GT, M=AC, N=ACGT, R=AG, S=GC, W=AT, Y=CT
Windows/Linux
In most browsers, pressing Ctrl+F5 will force the browser to retrieve the webpage.Mac
For Safari users on OS X, hold down ⌘ Cmd and ⇧ Shift key and then press R.Output: Export: Select all (Ctrl+A),
copy (Ctrl+C), paste (Ctrl+V)
Quick Help for LAMP Primer Sets
- Paste/upload FASTA or retrieve by NCBI accession, then tune length/Tm ranges for your chemistry.
- Use [ ... ] to constrain the target region and /.../ to exclude problematic segments.