Protocols

Curated laboratory protocols for DNA/RNA work and PCR workflows.

These pages provide practical step-by-step procedures. For software tools, see WebTools.

DNA extraction

CTAB and Proteinase K methods, including difficult tissues and herbarium/soil.

Open protocol

RNA extraction

Isolation of total RNA from plant/animal tissues.

Open protocol

Electrophoresis

Agarose gel electrophoresis for DNA/RNA, including denaturing options for RNA.

Open protocol

RNA/DNA preservation media

Preparation of preservation medium for nucleic acids.

Open protocol

PCR guide and troubleshooting

Best practices for template/primer concentrations and cycling optimization.

Open PCR guide