Primer & Probe Design Services

High-quality oligo design for research and diagnostics: from single assays to ultra-high multiplex panels.

Use our free online tools for quick drafts, or request an expert design package for validated, production-ready sets.

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What we design

PCR and multiplex PCR

  • Standard PCR, long-range PCR, and real-time PCR primer sets
  • Multiplex primer panels (interaction-aware design)
  • Re-design of existing primers to improve performance and specificity

qPCR probes and advanced chemistries

  • TaqMan® / MGB probe + primer sets (singleplex or multiplex)
  • Fluorescent primer options for quenching-based workflows
  • Assays with LNA / molecular beacons / Scorpion / related probes

Genotyping and variant assays

Isothermal and specialized workflows

  • LAMP assays (Eiken LAMP)
  • Gene walking (unknown flanks adjacent to a known sequence)
  • Overlap extension PCR (OE-PCR) multi-fragment assembly cloning
  • Polymerase Cycling Assembly (PCA) (Wikipedia)
  • MLPA® probe design (note)
  • XCR® primer design (technology)
We can design both overlapping and non-overlapping amplicon schemes, including tiling panels for targeted sequencing.

How projects are delivered

1

Requirements

Target sequences, organism/build, assay type, constraints (Tm/GC/length), multiplex level, and platform.

2

Design & QC

Specificity checks, interaction-aware multiplex design, and practical laboratory constraints.

3

Deliverables

Primer/probe lists, recommended conditions, and clear documentation for ordering and lab execution.

Pricing

Expert design services (€/design). For quick self-service drafts, use our free online tools.
Request a project quote
Product No. Description Price (€) per design
PCR Primer Design
4000 Design of 10–100 PCR primer pairs 5.0
4001 Design of >100 PCR primer pairs 4.0
4002 Design of 10–100 single forward or reverse primers 3.0
4003 Design of >100 single forward or reverse primers 2.5
4010 Design of 10–100 primer pairs for multiplex PCR 6.0
4011 Design of 1–100 primer pairs for LAMP 10.0
4020 Design of 1–100 single primers for repeats / PCR fingerprint 10.0
4003 Re-design of primer pairs to improve PCR efficiency 5.0
4100 Basic fee for design projects 100.0
Real-Time (qPCR) TaqMan/MGB Probe & Primer Design
5000 Design of 1–10 probes 10.0
5010 Design of >10 probes 7.0
5020 Design of 1–10 probes + appropriate primers for multiplex qPCR 10.0
5030 Design of 1–10 fluorescent primers for multiplex quenching-based qPCR 10.0
5100 Basic fee for design projects 100.0
Oligo Design for Microarrays
6000 Design of 1–100 oligos for microarrays 5.0
6010 Design of >100 oligos for microarrays 3.0
6100 Basic fee for design projects 100.0
Notes: Pricing is indicative for design tasks. Large or specialized projects may be quoted individually based on scope and constraints.

Ready to start?

Send targets (FASTA), organism/build, assay type, and constraints. We will reply with a recommended workflow and deliverables.

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