Business solutions
Practical PCR primer/probe design services and scientific software engineering to support R&D, diagnostics, and applied genomics projects.
We focus on deliverables that are directly usable in the lab or pipeline: validated primer/probe sets, project-ready designs, and robust tooling for high-throughput workflows.
Fast engagement
Send target sequences (FASTA/GenBank), organism/build, assay type (PCR/qPCR), and constraints (amplicon size,
multiplexing, chemistry). We respond with a plan and deliverable timeline.
What we deliver
Designed for science, diagnostics, forensic, and applied R&D
PCR
Primer and probe design
- General PCR primer design: for standard, inverted, long and quantitative PCR and sequencing applications.
- Design set of primers for multiplex PCR, the art multiplex primer design with PCR band size discrimination and other tasks without the need of PCR product size control.
- Design PCR primers for SSR loci, and applying for multiplex PCR.
- Re-design existing PCR primers.
- Design Group or unique specific primers for amplification of related sequences and detection of polymorphism or for cloning.
- Design primers for retrotransposon (or other repeats) detection polymorphisms.
qPCR
qPCR and microarray probe design
- Real-time PCR primer or probes (TaqMan, Molecular beacons, LUX Primers; Self-reporting primers and other).
- Microarray short oligonucleotides probe design.
- Re-design probes for important sequences to improving qPCR efficiency.
PM
Project services
- Developing efficient PCR primers for any PCR application in science, medicine, forensic or business.
- Multiplex PCR primers development project.
- PCR primers development for polymorphism detection for multi-locus genes project.
- Developing repeats based amplification polymorphism for any plant or animal genomes, base on simple repeats, retrotransposons, tandem repeats or other repeats.
- Project in-silico or laboratory experiments. Developing efficient PCR primers for detection polymorphism between individuals for any plant or animal genome.
- Probe design project.
DEV
Software development services
- Developing efficient algorithms for PCR primer and probe design for any applications, which use advanced algorithm searches for designing optimal primers and probes.
- Developing software for the customers needs.
Quality criteria
- Specificity assessment (off-target screening when a reference is available)
- Primer-dimer and secondary structure control for multiplex compatibility
- Platform- and chemistry-aware constraints (qPCR probes, LAMP, standard PCR)
- Deliverables include sequences, parameters, and usage notes
What to provide
- Target sequences or genomic regions (FASTA/GenBank)
- Organism / assembly (if applicable), expected variants and constraints
- Assay type (PCR/qPCR/SSR/LAMP), desired amplicon lengths
- Multiplexing goals (plex level, pools), sample context and inhibitors
Ready to start?
We can begin from sequences, primer lists, or a project brief.