Targeted Sequencing

We help teams move from biological question to sequencing-ready assay design-covering target definition, primer/probe design, multiplex optimization, and deliverables compatible with Illumina and Oxford Nanopore (ONT).

Best fit for

Pathogen panels and variant screening
Gene families and homologous loci
Structural variant breakpoints
Metagenomic targets (amplicon-style)

What we deliver

Panel design

Primer sets for single targets or multi-target panels, including tiling strategies.

In-silico specificity

Off-target and cross-mapping analysis against reference or custom databases.

Multiplex optimization

Interaction scanning, pool partitioning, and practical balancing notes.

Ordering-ready outputs

CSV/Excel tables with sequences, lengths, GC%, Tm, and amplicon coordinates.

Illumina workflows

Amplicon sizing for short-read constraints
Library compatibility and indexing considerations
Uniform coverage considerations across targets
Batch-friendly pool design for multi-sample studies

ONT workflows

Longer amplicons and tiled designs for genome coverage
Rapid vs ligation kit constraints and output templates
Robustness to indels and variable regions
Options for SV breakpoint validation and phasing targets

Typical engagement flow

Step 1

Define targets

FASTA/coordinates, organism/build, and success criteria.

Step 2

Design & filter

Thermodynamics, repeat-aware filtering, and candidate ranking.

Step 3

Validate in-silico

Mapping/off-target analysis and multiplex interaction checks.

Step 4

Deliverables

Ordering table + practical notes for wet-lab validation.

Note on validation

We provide design and computational validation. Wet-lab verification and any clinical/regulatory validation must be conducted by the customer according to local requirements.

Start a sequencing design request

Send targets (FASTA/coordinates), platform (Illumina/ONT), desired amplicon length, and sample context.