Virtual (in silico) or electronic PCR (ePCR) primer or probe search against whole genome(s) or a list of chromosomes - prediction of likely PCR products and search for potential mismatches of the specified primers or probes. Search for multiple targets simultaneously within a specified range. In silico oligonucleotide searching is useful for the discovery of target binding sites with melting temperature.
Degenerate primer sequences are also accepted. Each letter represents a combination of one or several nucleotides: B=(C,G,T), D=(A,G,T), H=(A,C,T), K=(G,T), M=(A,C), N=(A,C,G,T), R=(A,G), S=(G,C), V=(A,C,G), W=(A,T), Y=(C,T).
Upload or paste sequence(s) in FASTA format:Amplicon detection: | ||
Minimal amplicon size (bp): | ||
Maximal amplicon size (bp): | ||
Mismatches allowed in 3'-end: | ||