PCR reaction setup calculators

PCR and LAMP (Loop-mediated Isothermal Amplification) reaction setup calculator.

Standard PCR with Taq DNA Polymerase Phusion DNA Polymerase Phire Hot Start II DNA Polymerase Loop-mediated Isothermal Amplification (LAMP) Deep VentR DNA Polymerase KAPA HiFi DNA Polymerase Long PCR amplifications with Taq and Pfu DNA Polymerases Herculase II Fusion DNA Polymerases LongAmp Taq DNA Polymerase Pfu DNA Polymerase

Number of reactions:	x
Reaction volume:	µl
Total volume:	µl

	Concentration
	stock	final
			x
MgCl2 or MgSO4			mM
dNTP			mM
			µM
			µM
			µM
			µM
			µM
			µM
DMSO			%
ROX			x
SYBR Green II			x
			U/µl
Pfu DNA polymerase			U/µl
	DNA template		µl

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