Designing any
kind of PCR primers for standard, long distance, inverse, multiplex,
real-time, bisulphite modification assays, group-specific and
unique PCR analysis.
In silico (virtual) PCR primers and probes search -
prediction of probable PCR products and search of potential mismatching
location of the specified primer(s) or probe(s).
Analyzes different features of multiple primers simultaneously, e.g.
Tm, GC content, dimer formation and else.
The software also includes tools for identifying simple sequence repeat
(SSR) loci; automatically SSR loci detection and direct PCR primers
design.
Comprehensive primer test, Tm calculation, PCR efficiency, dimers detection and dilution and resuspension calculator.
Tool calculates the melting temperature for standard and degenerate oligonucleotides including LNA and other modifications. It can predict dimer formation and provides a dilution and resuspension calculator for stocks. Melting temperature calculations are based on nearest neighbour thermodynamic parameters.
Analyzes different features of multiple primers simultaneously, e.g. Tm, GC content, dimer formation and else; optimal Ta (temperature of annealing) calculation for all given primers.
The “PrimerList” tool provides analyses for a set of primers with prediction of oligonucleotide properties, self- and cross-dimer detection, and calculation of their melting temperatures.
PCR
and qPCR reaction setup calculator; tool for planning PCR and qPCR
reactions, mixing solutions.
Dilution calculator, applicable for mixing two solutions with various concentrations (molar or %, or other), as well as for various pH or mixing stock solution with solvent like water.