Primer Digital

Our world-class and complimentary probe design service is available for creating unique probes and primers specifically for most PCR applications and their combinations using machine learning (ML) approaches:

  • Standard, long distance and real-time PCR;
  • Multiplex PCR assay design;
  • Xtreme Chain Reaction (XCR®) primers design;
  • Gene walking project (finding unknown genomic DNA sequences adjacent to a known sequence);
  • Loop-mediated Isothermal Amplification (LAMP) assays design;
  • Group-specific (universal primers for genetically related DNA sequences) or unique (specific primers for each from genetically related DNA sequences);
  • MLPA® Probes or Multiplex Ligation-dependent Probe Amplification design service;
  • Kompetitive Allele Specific PCR (KASP™) or PACE™ (PCR Allele Competitive Extension) genotyping assay design and SNP/InDel markers design service;
  • The SNaPshot® Multiplex System assay for SNP markers design service;
  • The castPCR® - competitive, allele-specific TaqMan PCR utilizes an allele-specific primer for mutant allele detection that competes with an MGB blocker oligonucleotide to suppress the wild-type background and enable specific-allelic scoring of single nucleotide polymorphisms (SNPs) and insertions and deletions (InDels) at specific loci;
  • Design multiplexed of overlapping and non-overlapping DNA amplicons that tile across a region(s) of interest for targeted next-generation sequencing;
  • Simple sequence repeat (SSR) markers design;
  • Overlap extension PCR (OE-PCR) multi-fragments assembling cloning;
  • Single primer PCR (design of PCR primers from repeats: retrotransposons, etc);
  • Design multiplexed of overlapping and non-overlapping DNA amplicons that tile across a region(s) of interest for targeted next-generation sequencing (targeted gene sequencing panels);
  • Polymerase Chain Assembly (PCA) design service;
  • Singleplex and multiplex assays for dual-labeled probes (TaqMan® probes, TaqMan® MGB probes, LUX-primer, Scorpion and molecular beacons) and probes that contain LNA;

Design both overlapping and non-overlapping primers to generate either distinct or overlapping amplicons for whole genome amplification depending on the desired sensitivity.

Try our free probe design service to see how it can improve the success of your research.

Product-No Description Price [€] per Design
PCR Primer Design
4000 Design of 10 - 100 PCR primer pairs 5,0
4001 Design of > 100 PCR primer pairs 4,0
4002 Design of 10-100 single forward of reverse primer 3,0
4003 Design of > 100 single forward of reverse primer 2,5
4010 Design of 10 - 100 primer pairs for multiplex PCR 6,0
4011 Design of 1 - 100 primer pairs for LAMP 10,0
4020 Design of 1 - 100 single primer for repeats, PCR fingerprint 10,0
4003 Re-design primer pairs to improving PCR efficiency 5,0
4100 Basic fee for design projects 100,0
Real-Time (Quantitative) PCR TaqMan/MGB Probe and Primer Design
5000 Design of 1 - 10 probes 10,0
5010 Design of >10 probes 7,0
5020 Design of 1 - 10 probes and appropriate primers for multiplex real-time PCR 10,0
5030 Design of 1 - 10 appropriate fluorescent primers for multiplex quenching-based real-time PCR 10,0
5100 Basic fee for design projects 100,0
Oligo Design for Microarrays
6000 Design of 1 - 100 Oligos for microarrays 5,0
6010 Design of >100 Oligos for microarrays 3,0
6100 Basic fee for design projects 100,0

Contact us

PrimerDigital Ltd
c/o Helsinki Think Company Viikki
Latokartanonkaari 3
00790 Helsinki, Finland

  • Support contact
  • Business inquiries

Note: Product support is not available via telephone in any of the offices.
Free email accounts will not be accepted.