Primer Digital

Our world-class and complimentary probe design service is available for creating unique probes and primers specifically for most PCR applications and their combinations using machine learning (ML) approaches:

• Standard, long distance and real-time PCR;
• Multiplex PCR assay design;
• Xtreme Chain Reaction (XCR®) primers design;
• Gene walking project (finding unknown genomic DNA sequences adjacent to a known sequence);
• Loop-mediated Isothermal Amplification (LAMP) assays design;
• Group-specific (universal primers for genetically related DNA sequences) or unique (specific primers for each from genetically related DNA sequences);
• MLPA® Probes or Multiplex Ligation-dependent Probe Amplification design service;
• Kompetitive Allele Specific PCR (KASP™) or PACE™ (PCR Allele Competitive Extension) genotyping assay design and SNP/InDel markers design service;
• The SNaPshot® Multiplex System assay for SNP markers design service;
• The castPCR® - competitive, allele-specific TaqMan PCR utilizes an allele-specific primer for mutant allele detection that competes with an MGB blocker oligonucleotide to suppress the wild-type background and enable specific-allelic scoring of single nucleotide polymorphisms (SNPs) and insertions and deletions (InDels) at specific loci;
• Design multiplexed of overlapping and non-overlapping DNA amplicons that tile across a region(s) of interest for targeted next-generation sequencing;
• Simple sequence repeat (SSR) markers design;
• Overlap extension PCR (OE-PCR) multi-fragments assembling cloning;
• Single primer PCR (design of PCR primers from repeats: retrotransposons, etc);
• Design multiplexed of overlapping and non-overlapping DNA amplicons that tile across a region(s) of interest for targeted next-generation sequencing (targeted gene sequencing panels);
• Polymerase Chain Assembly (PCA) design service;
• Singleplex and multiplex assays for dual-labeled probes (TaqMan® probes, TaqMan® MGB probes, LUX-primer, Scorpion and molecular beacons) and probes that contain LNA;

Design both overlapping and non-overlapping primers to generate either distinct or overlapping amplicons for whole genome amplification depending on the desired sensitivity.

Try our free probe design service to see how it can improve the success of your research.

Product-No	Description	Price [€] per Design
	PCR Primer Design
4000	Design of 10 - 100 PCR primer pairs	5,0
4001	Design of > 100 PCR primer pairs	4,0
4002	Design of 10-100 single forward of reverse primer	3,0
4003	Design of > 100 single forward of reverse primer	2,5
4010	Design of 10 - 100 primer pairs for multiplex PCR	6,0
4011	Design of 1 - 100 primer pairs for LAMP	10,0
4020	Design of 1 - 100 single primer for repeats, PCR fingerprint	10,0
4003	Re-design primer pairs to improving PCR efficiency	5,0
4100	Basic fee for design projects	100,0
	Real-Time (Quantitative) PCR TaqMan/MGB Probe and Primer Design
5000	Design of 1 - 10 probes	10,0
5010	Design of >10 probes	7,0
5020	Design of 1 - 10 probes and appropriate primers for multiplex real-time PCR	10,0
5030	Design of 1 - 10 appropriate fluorescent primers for multiplex quenching-based real-time PCR	10,0
5100	Basic fee for design projects	100,0
	Oligo Design for Microarrays
6000	Design of 1 - 100 Oligos for microarrays	5,0
6010	Design of >100 Oligos for microarrays	3,0
6100	Basic fee for design projects	100,0