FastPCR is an integrated tool for PCR primers or probe design, in silico PCR, oligonucleotide assembly and analyses, alignment and repeat searching

The FastPCR software is an integrated tools environment that provides comprehensive facilities for designing primers for most PCR applications including standard, long distance, inverse, real-time, multiplex, unique and group-specific; overlap extension PCR (OE-PCR) multi-fragments assembling cloning; single primer PCR (design of PCR primers from close located inverted repeat), automatically SSR loci detection and direct PCR primers design, amino acid sequence degenerate PCR, Polymerase Chain Assembly (PCA) or oligos assembly and much more.
The “in silico” (virtual) PCR primers or probe searching, comprehensive pairs and individual primers analysis tests are included. The “in silico” oligonucleotide search is helpful for discovering target binding sites with the temperature melting calculation.
FastPCR has the capacity to handle long sequences and sets of nucleic acid or protein sequences and it allowed the individual task and parameters for each given sequences and joining several different tasks for single run. It also allows sequence editing and databases analysis. Efficient and complete detection of various types of repeats developed and applied to the program with a visualisation. 
The program includes various bioinformatics tools for analysis of sequences with GC or AT skew, CG content and purine-pyrimidine skew, the linguistic sequence complexity; generation random DNA sequence, restriction analysis and supports the clustering of sequences and consensus sequence generation and sequences similarity and conservancy analysis. 

System Requirements

  • Microsoft Windows 7/Vista/2003/XP/2000 (32-Bit (x86) or 64-Bit)
  • 2 GB RAM
  • 1280x800 minimum screen resolution

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Guided Video Tour of the FastPCR



PCR Primer Design Online Tool

PrimerDigital offers an online PCR primer design tool which capable you to define specific primers or probes easily by yourself.
In silico PCR primers and probes search and analyzes different features of multiple primers simultaneously, e.g. Tm, GC content, dimer formation and else. jPCR WebTools (FastPCR Online)

Comprehensive primer test

Tm calculation, PCR efficiency, dimers detection and dilution and resuspension calculator.
Tool calculates the melting temperature for standard and degenerate oligonucleotides including LNA and other modifications. jPCR WebTool

Oligos assembly

Polymerase chain assembly (PCA) or oligos assembly - created to automate the design oligonucleotide sets for long sequence assembly by ligase chain reaction (LCR) and PCR. jPCR WebTool

PCR reaction setup calculators

PCR and qPCR reaction setup calculator; tool for planning PCR and qPCR reactions, mixing solutions. PCR Setup WebTool

The licenses are available for users who wish to using PrimerDigital software products or engage in commercial uses, for clarification or further information regarding FastPCR and Web Tools licensing, contact us, at support@primerdigital.com